Coding

Part:BBa_K4759037:Design

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


TF-linker-OleP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 505
    Illegal AgeI site found at 1342
    Illegal AgeI site found at 1440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 73
    Illegal SapI.rc site found at 721
    Illegal SapI.rc site found at 834
    Illegal SapI.rc site found at 1090


Design Notes

TF via linker at the N terminus of Olep


Source

fusion tag

References